8/3/2023 0 Comments Smartscope fold microscopeThere are several examples in the literature of super-resolution under cryogenic conditions. If this could be overcome, the use of cryo-fixation could become routine in super-resolution microscopy, with the added benefit that rapid freezing is more effective than chemical fixation at preserving ultrastructure and minimizing artefacts, as demonstrated by electron microscopy (EM) 8. Liquid media freeze at cryogenic temperatures, so dry objectives (NA ≤ 0.9) are mostly used in this case, with the disadvantage of lower resolution. NAs >1 and preferably >1.4 are required to achieve high resolution, but these need immersion fluids to couple the sample to the objective. This depends on the number of photons emitted by the sample, which substantially increases under cryogenic conditions, the number of photons collected by the objective lens, and its numerical aperture (NA) 6, 7. In SMLM, resolution depends on the precision with which individual molecules can be localized 6, 7. Theoretically, resolution can be improved substantially by exploiting SMLM techniques under cryogenic conditions. However, when the goal is to ascertain structure–function relationships, the challenge is to improve resolution by at least ~2-fold, making it comparable with the typical size of interacting macro-molecular units (~10 nm). Techniques such as structured illumination microscopy (SIM) 1, stimulated emission depletion (STED) microscopy 2, and single molecule localization microscopy (SMLM) (including stochastic optical reconstruction microscopy (STORM) 3, photoactivated localization microscopy (PALM) 4, and fluorescence photoactivated localization microscopy (FPALM) 5) image cellular processes at resolutions in the 20–100 nm range. Super-resolution fluorescence microscopy has underpinned our understanding of interacting molecular networks in cells. To fully understand the cellular function, we must combine cellular ultrastructure information with knowledge of protein interaction networks at the molecular level. By also allowing multicolour imaging, and by paving the way to total-internal-reflection fluorescence imaging of mammalian cells under cryogenic conditions, s uperSIL microscopy opens a straightforward route to achieve unmatched resolution on bacterial and mammalian cell samples. Here, using a low-cost super-hemispherical solid immersion lens ( superSIL) and a basic set-up we achieve 12 nm resolution under cryogenic conditions, to our knowledge the best yet attained in cells using simple set-ups and/or commercial systems. Cryogenic conditions are, however, underutilised because of the lack of compatible high numerical aperture objectives. This substantially increases the brightness of most fluorophores and preserves native ultrastructure much better than chemical fixation. A path to improve resolution is to image under cryogenic conditions. However, this resolution is insufficient to image macro-molecular machinery at work. Or you can simply stream a live video directly on your TV (if your mobile phone allows screen mirroring).Super-resolution fluorescence microscopy plays a crucial role in our understanding of cell structure and function by reporting cellular ultrastructure with 20–30 nm resolution. Thanks to high quality optics, the image quality is so amazing that you can even do further digital zoom to increase the magnification. All the demo pictures and videos have been shot with the above combination. It has the perfect magnification to enjoy watching the amazing world of microorganisms. Dimensions: Length 140 mm, Width 55 mm, Height 140 mm. Comes as a complete set which includes a carry case and prepared slides.Long lasting battery life (over a year with normal usage).High quality optical parts as found in any professional microscope.Even small kids can easily use it on their own. You can simply use your smartphone screen to view the enlarged image and click pictures and videos. It's great fun to use and has an amazing educational value for kids, students or teachers. It's a complete redesign of the classical field microscope. It's a small optical microscope specially designed for Smartphones.
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